Two US B-52 bomber planes have flown near artificial islands claimed by China in the South China Sea, the Pentagon said.
Their mission continued despite being warned by Chinese ground controllers.
The incident comes ahead of a visit by US President Barack Obama to a summit in Manila next week, which China's President Xi Jinping will also attend.
China is locked in maritime territorial disputes with several neighbours in the South China Sea.
China claims a large swathe of the resource-rich area and has been aggressively reclaiming land and building facilities on reefs, which the US and others oppose.
The US has said it plans to demonstrate its freedom of navigation principle in the sea, which challenges what it deems to be "excessive claims" to the world's oceans and airspace.
'Received warnings'The latest US patrol, which took place overnight on Sunday near the Spratly Islands, was a "a routine mission in the SCS (South China Sea)", said Pentagon spokesman Bill Urban, taking off from Guam and returning there.
The B-52s did not breach the area claimed by China around the islands, the Pentagon said.
Mr Urban told reporters that the planes received two warnings "despite never venturing within 15 nautical miles of any feature".
"Both aircraft continued their mission without incident, and at all times operated fully in accordance with international law," he said.
Last month, the US sailed warship USS Lassen into the 12-nautical mile zone China claims around Subi and Mischief reefs in the Spratly archipelago, angering Beijing.
China's foreign ministry condemned it as "illegal".
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